This project has been funded by NSF award #
0501857
(PIs: Y. Yu, C. Soderlund, V. Walbot), which
will generate ~30,000 maize FLcDNA sequences.
The project
will use genomics tools to understand a fundamental biological process
through identificaion of genes expressed during maize reproduction and in somatic tissues
responding to abiotic perturbations such as heat, cold, salt, UV-B, drought, and lack of light.
See objectives for detailed information.
Points of Interest
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- We have submitted 366,390 ESTs to
Genbank (Aug 06):
- Library #1 has 243767 reads of which 209140 are paired reads.
Average length after trimming is 663 bases.
- Library #2 has 122622 reads of which 109350 are paired reads.
Average length after trimming is 731 bases.
- See Outreach Tutorial, which includes:
- FLcDNA Tutorial, Why FLcDNA, Transgenic Plants
- Approach, Glossary, Resources
- The PAVE EST assembly (July 07):
- The assembly contains 705,119 maize ESTs from Genbank,
which have passed the filtering
at PlantGDB.
- 87,592 contigs and 114,709 singletons.
- Due to a problem in trimming the ESTs for this project, a new assembly is in progress - coming soon...
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